Comparison of the phenotypic profile of antimicrobial resistance in the oral microbiota of non-smokers, tobacco smokers, and electronic cigarette vapers - A pilot study

Authors

  • Enrique Alejandro Martínez-Solís Universidad Anáhuac México, Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Salud (CICSA) https://orcid.org/0000-0002-5563-8891
  • Abraham Atman Hernández-Martínez Universidad Anáhuac México, Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Salud (CICSA), Departamento de Microbiología https://orcid.org/0000-0003-2452-4119
  • Adán Peña-Barreto Universidad Anáhuac México, Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Salud (CICSA), Departamento de Microbiología https://orcid.org/0000-0002-7489-5324
  • Tomás Barrientos-Fortes Universidad Anáhuac México, Sistema Universitario de Salud https://orcid.org/0000-0001-6930-3710
  • Diego Abelardo Álvarez-Hernández Universidad Anáhuac México, Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Salud (CICSA), Departamento de Microbiología https://orcid.org/0000-0003-2340-6639
  • Sandra Georgina Solano-Gálvez  Universidad Nacional Autónoma de México, Facultad de Medicina, Departamento de Microbiología https://orcid.org/0000-0003-0845-7200
  • Rosalino Vázquez-López Universidad Anáhuac México, Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Salud (CICSA), Departamento de Microbiología https://orcid.org/0000-0002-1982-7329

DOI:

https://doi.org/10.36105/psrua.2021v1n1.01

Keywords:

cigarrillo, cigarrillos electrónicos, vapear, microbiota, resistencia a fármacos antimicrobianos

Abstract

Introduction: The microbiota is a community of microorganisms that live in a specific environment. Their type and number depend on multiple internal and external factors. Oral is the second most diverse and populated microbiota of the body. Smoking and vaping induce changes in its composition, and it has been demonstrated that they can lead to an increase in antimicrobial resistance. Objective: To compare the phenotypic profile of antimicrobial resistance in the oral microbiota of non-smokers, tobacco users, and electronic cigarette vapers. Methods: An observational, descriptive, cross-sectional, and comparative study was carried out. Three groups of non-smokers, smokers of conventional tobacco, and electronic cigarette (EC) vapers of tobacco flavored e-juice were formed. Oral cavity samples were obtained, incubated, and seeded in agar plates. Bacteria were isolated and identified performing Gram staining, oxidase, indole, and biochemical test panels. Susceptibility tests were performed using a MicroScan autoSCAN-4 system and the Kirby–Bauer test. Results: Variation was observed in the populations of bacteria that were isolated in each of the groups, but the non-smokers showed the most pathogens. In the non-smoking group, Staphylococcus sciuri was the most common bacteria, Staphylococcus sciuri and Enterobacter cloacae were the most abundant in the smoking group, and in the EC vapers group, the most common bacteria were Staphylococcus epidermidis and Staphylococcus sciuri. Conclusion: Multidrug resistance was observed in all the groups. However, EC vapers showed the highest proportions of antimicrobial resistance, raising a major concern.

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References

1. Conrad, R.; Vlassov, A.V. The Human Microbiota: Composition, Functions, and Therapeutic Potential. Med Sci Rev. 2015; 2: 1-12. https://doi.org/10.12659/MSRev.895154
2. Turnbaugh, P.J.; Ley, R.E.; Hamady, M.; Fraser-Liggett, C.M.; Knight, R.; Gordon, J.I. The human microbiome project. Nature. 2007; 449 (7164): 804-10. https://dx.doi.org/10.1038/nature06244
3. Domínguez-Bello, M.G.; Godoy-Vitorino, F.; Kinght, R.; Blaser, M.J. Role of the microbiome in human development. BMJ. 2019; 0: 1-7. http://dx.doi.org/10.1136/gutjnl-2018-317503
4. Hasan, N.; Yang, H. Factors affecting the composition of the gut microbiota, and its modulation. PeerJ. 2019; 7: 7502. https://doi.org/10.7717/peerj.7502
5. Valles, Y.; Inman, C.K.; Peters, B.A.; Ali, R.; Wareth, L.A.; Abdulle, A. Types of tobacco consumption and the oral microbiome in the United Arab Emirates Healthy Future (UAEHFS) Pilot Study. Sci Rep. 2018; 8: 11327. https://doi.org/10.1038/s41598-018-29730-x
6. Roberts, A.P; Kreth, J. The impact of horizontal gene transfer on the adaptive ability of the human oral microbiome. Front Cell Infect Mi. 2014; 4: 124. https://doi.org/10.3389/fcimb.2014.00124
7. Sampaio-Maia, B.; Caldas, I.M.; Pereira, M.L.; Péres-Mongiovi, D.; Araujo, R. The Oral Microbiome in Health and Its Implication in Oral and Systemic Diseases. Adv Appl Microbiol. 2016; 97: 171-210. https://doi.org/10.1016/bs.aambs.2016.08.002
8. Qasim, H.; Karim, Z.A.; Rivera, J.O.; Khasawneh, F.T.; Alshbool, F.Z. Impact of Electronic Cigarettes on the Cardiovascular System. J Am Heart Assoc. 2017; 9: 6353. https://doi.org/10.1161/JAHA.117.006353
9. Brook, I.; Gober, A.E. Recovery of potential pathogens in the nasopharynx of health and otitis media-prone children and their smoking and nonsmoking parents. Ann Otol Rhinol Laryngol. 2008; 117: 727-730. https://doi.org/10.1177/000348940811701003
10. Brook, I.; Gober, A.E. Recovery of potential pathogens and interfering bacteria in the nasopharynx of smokers and nonsmokers. Chest J. 2005; 127: 2072-2075. https://doi.org/10.1177/000348940811701003
11. Lacoma, A.; Edwards, A.M.; Young, B.C.; Domínguez, J.; Prat, C.; Laabei, M. Cigarette smoke exposure redirects Staphylococcus aureus to avirulence profile associated with persistent infection. Sci Rep. 2019; 9: 1-15. https://doi.org/10.1038/s41598-019-47258-6
12. Layden, J.E.; Ghinai, I.; Pray, I.; Kimbal, A.; Layer, M., Tenforde, M, et al. Pulmonary Illness Related to E-Cigarette Use in Illinois and Wisconsin — Preliminary Report. N Engl J Med. 2019; In press. https://doi.org/10.1056/NEJMoa1911614
13. Campos, M.A.; Guerrero, A.M.; Calderon-Candelario, R.A.; Holt, G.E.; Mirsaeidi, M.; Mendez, R. Oral Microbiome Differences Associated with Replacing Tobacco Smoking with Electronic Cigarette Vaping. Am J Respir Crit Care Med. 2019; 199: 7428. https://doi.org/10.1164/ajrccm-conference.2019.199.1_MeetingAbstracts.A7428
14. Stewart, C.J.; Auchtung, T.A.; Ajami, N.J.; Velásquez, K.; Smith, D.P.; de la Garza II, R, et al. Effects of tobacco smoke and electronic cigarette vapor exposure on the oral and gut microbiota in humans: a pilot study. PeerJ. 2018; 6: 4693. https://doi.org/10.7717/peerj.4693
15. Kilian, M.; Chapple, I.L.C.; Hanning, M.; Marsch, P.D.; Meuric, V.; Pedersen, A.M.L.; et al. The oral microbiome – an update for oral healthcare professionals. Brit Dental J. 2016; 221 (1): 657-666. https://doi.org/10.1038/sj.bdj.2016.865
16. Rinninella, E.; Raoul, P.; Cintoni, M.; Franceschi, F.; Donato-Miggiano, G.A.; Gasbarrini, A.; Mele, M.C. What is the Healthy Gut Microbiota Composition? A Changing Ecosystem across Age, Environment, Diet, and Diseases. Microorganisms. 2019; 7 (1): 14. https://doi.org/10.3390/microorganisms7010014
17. Sampaio-Maia, B.; Monteiro-Silva, F. Acquisition and maturation of oral microbiome throughout childhood: An update. Dent Res J. 2014; 11: 291-301.
18. Human Microbiome Project Consortium. A framework for human microbiome research. Nature. 2012; 486: 215-221. https://doi.org/10.1038/nature11209
19. Marsh PD. Role of the oral microflora in health. Microbial Ecol Health Dis. 2009; 12: 130-137. https://doi.org/10.1080/089106000750051800
20. Sharma, N.; Bhatia, S.; Singh-Sodhi, A.; Batra, N. Oral microbiome and health. AIMS Microbiology. 2018; 4 (1): 42-46. https://doi.org/10.1080/089106000750051800
21. Lee, S.H.; Yun, Y.; Kim, S.J.; Lee, E.-J.; Chang, Y.; Ryu, S.; et al. Association between Cigarette Smoking Status and Composition of Gut Microbiota: Population-Based Cross-Sectional Study. J Clin Med. 2018; 7 (9): 282. https://doi.org/10.3390/jcm7090282
22. Yu, G.; Phillips, S.; Gail, M.H.; Goedert, J.J.; Humphrys, M.S.; Ravel, J.; et al. The effect of cigarette smoking on the oral and nasal microbiota. Microbiome. 2017; 5: 3. https://doi.org/10.1186/s40168-016-0226-6
23. Shen, P.; Whelan, F.J.; Schenck, L.P.; McGrath, J.J.C.; Vanderstocken, G.; Bowdish, D.M.E.; et al. Streptococcus pneumoniae Colonization Is Re-quired To Alter the Nasal Microbiota in Cigarette Smoke-Exposed Mice. Infect Immun. 2017; 85 (10): 1-14.
https://doi.org/10.1186/s40168-016-0226-6
24. Cuadra, G.A.; Smith, M.T.; Nelson, J.M.; Loh, E.K.; Palazzolo, D.L. A Comparison of Flavorless Electronic Cigarette-Generated Aerosol and Conventional Cigarette Smoke on the Survival and Growth of Common Oral Commensal Streptococci. Int J Environ Res Public Health. 2019; 16: 1669. https://doi.org/I10.1128/IAI.00434-17
25. Chopyk, J.; Chattopadhyay, S.; Kulkarni, P.; Claye, E.; Babik, K.R.; Reid, M.C.; et al. Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens. Microbiome. 2017; 5: 22. https://doi.org/10.1186/s40168-017-0235-0
26. Kim, S.A.; Smith, S.; Beauchamp, C.; Song, Y.; Chiang, M.; Giuseppetti, A.; et al. Cariogenic potential of sweet flavors in electronic-cigarette liquids. PLoS One. 2018; 13 (9): 1-22. https://doi.org/10.1371/journal.pone.0203717
27. Vivas, R.; Teixeira Barbosa, A.; Multidrug-Resistant Bacteria and Alternative Methods to Control Them: An Overview. Mary Ann Liebert, Inc., publishers. 2019. https://doi.org/10.1089/mdr.2018.0319
28. Capozzi, C.; Maurici, M.; Panà, A.; Antimicrobico resistenza: è crisi globale, “un lento tsunami” [Antimicrobial resistance: it is a global crisis, "a slow tsunami"]. Ig Sanita Pubbl. 2019; 75(6):429-450.
29. Kassinen A.; Krogius-Kurikka L.; Mäkivuokko H.; Rinttilä T.; Paulin L.; Corander J.; et al. The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects. NCBI. 2007; 133(1): 24-33. https://doi.org/10.1053/j.gastro.2007.04.005

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Published

2021-07-22

How to Cite

Martínez-Solís, E. A., Hernández-Martínez, A. A., Peña-Barreto, A., Barrientos-Fortes, T., Álvarez-Hernández, D. A., Solano-Gálvez, S. G., & Vázquez-López, R. (2021). Comparison of the phenotypic profile of antimicrobial resistance in the oral microbiota of non-smokers, tobacco smokers, and electronic cigarette vapers - A pilot study. Proceedings of Scientific Research Universidad Anáhuac. Multidisciplinary Journal of Healthcare, 1(1), 5–13. https://doi.org/10.36105/psrua.2021v1n1.01

Issue

Vol. 1 No. 1 (2021): Proceedings of Scientific Research Universidad Anáhuac

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Original Research

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Copyright (c) 2021 Enrique Alejandro Martínez-Solís, Abraham Atman Hernández-Martínez, Adán Peña-Barreto, Tomás Barrientos-Fortes, Diego Abelardo Álvarez-Hernández, Sandra Georgina Solano-Gálvez, Rosalino Vázquez-López

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